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Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker
Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker from Abcam
(1)
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Citations:
(125)
Supplier Page
Supplier Page from
Abcam for
Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab11826
Antibody Type
Primary
Clone
SC-35
Isotype
IgG1
Host
Mouse, Nude mouse
Clonality
Monoclonal
Quantity
10 UG, 100 UG
Applications
Affinity purification, Immunocytochemistry/ Immunofluorescence, Affinity purification, Immunocytochemistry/ Immunofluorescence
Conjugate/Tag
Unconjugated
Concentration
1 mg/ml
Gene Name
SRSF2
Reactivity
Human, Mouse, Rat
Target
SC35
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (125)
(61)
SAF-A Regulates Interphase Chromosome Structure through Oligomerization with Chromatin-Associated RNAs
Cell
June 15, 2017
Nozawa RS, Boteva L, Soares DC, Naughton C, Dun AR, Buckle A, Ramsahoye B, Bruton PC, Saleeb RS, Arnedo M,
et al
.
immunofluorescence
),
Abcam
,Cat#
ab15580
; RRID: AB_443209
anti-sc35
(
1:300
for
immunofluorescence
),
Abcam
,Cat#
ab11826
; RRID: AB_298608 anti-BLM (
1:500
for
immunofluorescence
),
Santa Cruz
,Cat#
sc7790
; RRID: AB_2243489 "Goat
(62)
RNA phase transitions in repeat expansion disorders
Nature
June 8, 2017
Ankur Jain, Ronald D. Vale
antibodies against muscleblind-like-1 (MBNL1,
Abcam
,
ab45899
), hnRNP H (
Abcam
,
ab10374
), SC-35 (
Abcam
,
ab11826
), Coilin (
ab87913
), Fibrillarin (
Abcam
,
ab5821
) and
PML
(
Abcam
,
ab179466
) and a corresponding Alexa
(63)
Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex
Nature communications
May 25, 2017
Teng T, Tsai JH, Puyang X, Seiler M, Peng S, Prajapati S, Aird D, Buonamici S, Caleb B, Chan B,
et al
.
incubated with α-
SF3B1
mouse
monoclonal antibody (MBL,
D221-3
) or α-SC35
mouse
monoclonal antibody (
Abcam
,
ab11826
) at
1:50
dilution in 5% FBS/PBS in cold room overnight. On the second day, coverslips were washed with
(64)
Differential Targeting of Hsp70 Heat Shock Proteins HSPA6 and HSPA1A with Components of a Protein Disaggregation/Refolding Machine in Differentiated Human Neuronal Cells following Thermal Stress
Frontiers in Neuroscience
April 24, 2017
Catherine A. S. Deane, Ian R. Brown
Sciences (Farmingdale, NY, USA). DNAJA1 [
clone
KA2A5.6] (
ab3089
),
HSPH1
(
ab109624
), BAG-1 (
ab7976
), SC35 (
ab11826
), nucleophosmin (
ab37659
), and RNA polymerase II CTD repeat YSPTSPS (phospho S5) (
ab5131
) primary antibodies
(65)
A Cytoplasmic RNA Virus Alters the Function of the Cell Splicing Protein SRSF2
Journal of virology
April 1, 2017
Rivera-Serrano EE, Fritch EJ, Scholl EH, Sherry B.
immunofluorescence and PLA were anti-coilin (
Cell Signaling Technology
,
14168
;
1:800
), anti-SRSF2/SC35 (
Abcam
,
ab11826
;
1:1,000
),
anti-Son
(
Abcam
,
ab109472
;
1:200
), anti-HA epitope tag (GeneTex, 18181;
1:1200
; or
Sigma
,
(66)
C9ORF72 hexanucleotide repeat exerts toxicity in a stable, inducible motor neuronal cell model, which is rescued by partial depletion of Pten
Human molecular genetics
March 15, 2017
Stopford MJ, Higginbottom A, Hautbergue GM, Cooper-Knock J, Mulcahy PJ, De Vos KJ, Renton AE, Pliner H, Calvo A, Chio A,
et al
.
rabbit polyclonal anti-SRSF1 (1 in 200,
Abcam
ab38017
),
mouse
monoclonal anti-SRSF2 (1 in 200,
Abcam
ab11826
), or rabbit polyclonal
anti-NCL
(
1:200
;
Proteintech
;
10556-1-AP
) in 2% BSA in PBS at RT for 1 h. Slides
(67)
Components of a mammalian protein disaggregation/refolding machine are targeted to nuclear speckles following thermal stress in differentiated human neuronal cells
Cell Stress and Chaperones
March 1, 2017
Catherine A. S. Deane, Ian R. Brown
Sciences (Farmingdale, NY, USA). DNAJA1 [
clone
KA2A5.6] (
ab3089
),
HSPH1
(
ab109624
), BAG-1 (
ab7976
), SC35 (
ab11826
), and nucleophosmin (
ab37659
) primary antibodies were purchased from
Abcam
(Toronto, ON, CA). Primary
(68)
Diabetic polyneuropathy, sensory neurons, nuclear structure and spliceosome alterations: a role for CWC22
Disease models & mechanisms
March 1, 2017
Kobayashi M, Chandrasekhar A, Cheng C, Martinez JA, Ng H, de la Hoz C, Zochodne DW.
Santa Cruz Biotechnology
) for CBs,
mouse
anti-SMN
(
1:200
;
NB100-1936
,
Novus Biologicals
),
mouse
anti-SC35
(
1:500
;
ab11826
,
Abcam
) for
nuclear speckles
, rabbit
anti-CWC22
(
1:100
;
HPA036748
,
Sigma-Aldrich
),
rabbit-anti-TARDBP antibody
(69)
Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6
Scientific reports
February 20, 2017
Huang JH, Ku WC, Chen YC, Chang YL, Chu CY.
Probe set, following the manufacturer’s instructions. For anti-
DDX6
and anti-phosphorylated SC35 IF (
ab11826
,
Abcam
) after MALAT1 FISH, the cells were washed with 50% FISH wash buffer in PBS, PBS and 0.1% Triton
(70)
miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif
Frontiers in molecular neuroscience
January 1, 2017
Goldie BJ, Fitzsimmons C, Weidenhofer J, Atkins JR, Wang DO, Cairns MJ.
rabbit anti-
Ago1
(
ProteinTech
19690-1-AP
), rabbit anti-Ago2 (
Abcam
ab156970
), and
mouse
anti-SC35
(
Abcam
ab11826
) was overnight at 4°C. Appropriate secondary antibodies were selected from the Alexa-color series (Invitrogen).
(71)
Nuclear bodies reorganize during myogenesis in vitro and are differentially disrupted by expression of FSHD-associated DUX4
Skeletal muscle
December 1, 2016
Homma S, Beermann ML, Yu B, Boyce FM, Miller JB.
(cat.
sc-966
,
Santa Cruz
Biotech, Dallas TX) used at
1:200
. SC35 was detected with a
mouse
mAb (cat.
ab11826
, lot GR272322-1;
Abcam
) used at
1:1000
.
FUS
was detected with a rabbit pAb (cat.
11570-1-AP
, lot 00024677;
(72)
Profiling stem cell states in three-dimensional biomaterial niches using high content image informatics
Acta biomaterialia
November 1, 2016
Dhaliwal A, Brenner M, Wolujewicz P, Zhang Z, Mao Y, Batish M, Kohn J, Moghe PV.
Immuno-
fluorescence
staining
for SC-35,
mouse
monoclonal antibody specific to SC35 (
Abcam
, catalog number
ab11826
) was used as the primary antibody and Goat anti-Mouse IgG1 conjugated to Alexa Fluor 488 (LifeTechnologies,
(73)
Toxic PR Poly-Dipeptides Encoded by the C9orf72 Repeat Expansion Target LC Domain Polymers
Cell
October 20, 2016
Yi Lin, Eiichiro Mori, Masato Kato, Siheng Xiang, Leeju Wu, Ilmin Kwon, Steven L. McKnight
(74)
Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility
Open biology
September 1, 2016
Taylor SE, Bagnall J, Mason D, Levy R, Fernig DG, See V.
mouse
anti-RNAPII phospho ser5 (
1 : 50
; #
ab24759
,
Abcam
, Cambridge, UK),
mouse
anti-SC35
(
1 : 1000
; #
ab11826
,
Abcam
, Cambridge, UK),
mouse
anti-Sart1 (10 µg ml−1; #
ab88583
,
Abcam
, Cambridge, UK), anti-rabbit Alexa
(75)
Myelodysplasia-associated mutations in serine/arginine-rich splicing factor SRSF2 lead to alternative splicing of CDC25C
BMC molecular biology
August 23, 2016
Skrdlant L, Stark JM, Lin RJ.
were incubated with primary antibodies [rabbit anti-HA tag (Abcam;
ab9110
),
mouse
anti-SC35
(
Abcam
;
ab11826
), and
mouse
anti-γH2AX (
ab11174
)] each diluted
1:100
in the blocking solution for 1 h at RT. Coverslips
(76)
Severe muscle wasting and denervation in mice lacking the RNA-binding protein ZFP106
Proceedings of the National Academy of Sciences of the United States of America
August 2, 2016
Anderson DM, Cannavino J, Li H, Anderson KM, Nelson BR, McAnally J, Bezprozvannaya S, Liu Y, Lin W, Liu N,
et al
.
1% Triton X-100 in PBS for 15 min at room temperature.
Immunofluorescence
staining
for SC-35 (
Abcam
;
ab11826
), GFAP (
Abcam
;
ab7260
),
Iba1
(
Abcam
;
ab107159
), TDP-43 (Proteintech; 10782-1-AP), FUS (
Abcam
;
ab70381
),
(77)
Higher-order oligomerization promotes localization of SPOP to liquid nuclear speckles
The EMBO journal
June 15, 2016
Marzahn MR, Marada S, Lee J, Nourse A, Kenrick S, Zhao H, Ben-Nissan G, Kolaitis RM, Peters JL, Pounds S,
et al
.
visualize SPOP,
GFP
fluorescence
was used to visualize Gli3,
mouse
anti‐SC‐35 antibody (
1:250
;
Abcam
, cat#
ab11826
), rabbit anti‐coilin antibody (
1:200
;
Santa Cruz Biotechnology
, cat#
SC32860
),
mouse
anti‐B23 antibody
(78)
Pur-Alpha Induces JCV Gene Expression and Viral Replication by Suppressing SRSF1 in Glial Cells
PLOS ONE
June 3, 2016
Ilker Kudret Sariyer, Rahsan Sariyer, Jessica Otte, Jennifer Gordon
(pAb416, Calbiochem),
mouse
monoclonal antiSRSF1 (
Clone
96,
Invitrogen
),
mouse
monoclonal
anti-SRSF2
(
Ab11826
,
Abcam
), and rabbit polyclonal anti-SRSF3 (HPA056981, Sigma-Aldrich).
Mouse
monoclonal anti-GRB2 antibody (
clone
(79)
Genome-wide lentiviral shRNA screen identifies serine/arginine-rich splicing factor 2 as a determinant of oncolytic virus activity in breast cancer cells
Oncogene
May 12, 2016
Workenhe ST, Ketela T, Moffat J, Cuddington BP, Mossman KL.
USA) (cat. no.
556363
). The antibody used to detect phospho-
SRSF2
was obtained from
Abcam
(cat. no.
ab11826
; Cambridge, MA, USA). The
mouse
anti-HSV-1 gB was obtained from Virusys Corporation (Taneytown, MD, USA).
(80)
Sequencing of FTO and ALKBH5 in men undergoing infertility work-up identifies an infertility-associated variant and two missense mutations
Fertility and sterility
May 1, 2016
Miriam Landfors, Sigve Nakken, Markus Fusser, John-Arne Dahl, Arne Klungland, Peter Fedorcsak
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Reviews
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(1)
12/28/2016
Good Antibody For Nuclear Speckles
Tara Vanderweyde
We used this antibody to assess which nuclear substructure was associating with our protein of interest. Antibody worked well at indicated concentrations in rat and mouse cell lines for ...
Read Review
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Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker from Abcam
Supplier Page
View Company Product Page
Pricing Info
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Company Info
Citations
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